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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads
 Plucnara, Medard ; Havran, Luděk ; Ecsin, E. ; Erdem, A. ; Fojta, Miroslav
Novel approach of SNP detection in model synthetic oligonucleotides is described.This assay requires only intrinsic electrochemical properties of DNA and except of incorporation of biotin affinity tag, no any labeling or any other modification step is needed. Very good resolution for different variants of polymorphism as well as sufficiently low detection limits were achieved.In combination with using pencil graphited electrode this assay could be very simple and cheap tool for SNP typing.
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